High-throughput RNA sequencing reveals the effects of 2,2′,4,4′ -tetrabromodiphenyl ether on retina and bone development of zebrafish larvae
Identifieur interne : 000031 ( Main/Exploration ); précédent : 000030; suivant : 000032High-throughput RNA sequencing reveals the effects of 2,2′,4,4′ -tetrabromodiphenyl ether on retina and bone development of zebrafish larvae
Auteurs : Ting Xu [République populaire de Chine] ; Jing Zhao [République populaire de Chine] ; Daqiang Yin [République populaire de Chine] ; Qingshun Zhao [République populaire de Chine] ; Bingzhi Dong [République populaire de Chine]Source :
- BMC Genomics [ 1471-2164 ] ; 2015.
Abstract
2,2′,4,4′-Tetrabromodiphenyl ether (BDE47) is a prevalent environmental pollutant and has been demonstrated to be a serious toxicant in both humans and animals, but little is known about the molecular mechanism underlying its toxic effect on the early development of vertebrates. BDE47-treated zebrafish larvae were found to present the light-related locomotion reduction in our previous study, therefore, we aimed to use high throughput sequencing to investigate the possible reasons from a transcriptomic perspective.
By exposing zebrafish embryos/larvae to 5 μg/l and 500 μg/l BDE47, we measured the influence of BDE47 on the mRNA expression profiles of zebrafish larvae until 6 days post-fertilization, using Illumina HiSeq 2000 sequencing. Differential expression analysis and gene enrichment analysis respectively revealed that a great number of genes, and gene sets based on two popular terminologies, were affected by the treatment of 500 μg/l BDE47. Among them, BDE47 caused changes in the retinal metabolism and related biological processes involving eye morphogenesis and visual perception, as confirmed by disordered photoreceptor arrangement and thickened bipolar cell layer of larval retina from histological observations. Other altered genes such as
Our results suggest that high BDE47 concentrations disrupt the eye and bone development of zebrafish larvae based on both transcriptomic and morphological evidences. The abnormal visual perception may result in the alteration of dark adaption, which was probably responsible for the abnormal larval locomotion. Body curvature arose from enhanced bone resorption because of the intensive up-regulation of related genes. We also proposed the larval retina as a novel potential target tissue for BDE47 exposure.
The online version of this article (doi:10.1186/s12864-014-1194-5) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s12864-014-1194-5
PubMed: 25614096
PubMed Central: 4312473
Affiliations:
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College of Environmental Science and Technology, Tongji University, Shanghai, 200092 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Technology, Tongji University, Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Zhao, Qingshun" sort="Zhao, Qingshun" uniqKey="Zhao Q" first="Qingshun" last="Zhao">Qingshun Zhao</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Model Animal Research Center, MOE Key Laboratory of Model Animal for Disease Study, Nanjing University, Nanjing, 210061 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Model Animal Research Center, MOE Key Laboratory of Model Animal for Disease Study, Nanjing University, Nanjing</wicri:regionArea></affiliation></author><author><name sortKey="Dong, Bingzhi" sort="Dong, Bingzhi" uniqKey="Dong B" 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xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Post-doctoral Research Station of Civil Engineering, Tongji University, Shanghai</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2">Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Technology, Tongji University, Shanghai, 200092 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Technology, Tongji University, Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Zhao, Jing" sort="Zhao, Jing" uniqKey="Zhao J" first="Jing" last="Zhao">Jing Zhao</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Technology, Tongji University, Shanghai, 200092 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Technology, Tongji University, Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Yin, Daqiang" sort="Yin, Daqiang" uniqKey="Yin D" first="Daqiang" last="Yin">Daqiang Yin</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Technology, Tongji University, Shanghai, 200092 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Technology, Tongji University, Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Zhao, Qingshun" sort="Zhao, Qingshun" uniqKey="Zhao Q" first="Qingshun" last="Zhao">Qingshun Zhao</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Model Animal Research Center, MOE Key Laboratory of Model Animal for Disease Study, Nanjing University, Nanjing, 210061 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Model Animal Research Center, MOE Key Laboratory of Model Animal for Disease Study, Nanjing University, Nanjing</wicri:regionArea></affiliation></author><author><name sortKey="Dong, Bingzhi" sort="Dong, Bingzhi" uniqKey="Dong B" first="Bingzhi" last="Dong">Bingzhi Dong</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Post-doctoral Research Station of Civil Engineering, Tongji University, Shanghai, 200092 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Post-doctoral Research Station of Civil Engineering, Tongji University, Shanghai</wicri:regionArea></affiliation></author></analytic><series><title level="j">BMC Genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>2,2′,4,4′-Tetrabromodiphenyl ether (BDE47) is a prevalent environmental pollutant and has been demonstrated to be a serious toxicant in both humans and animals, but little is known about the molecular mechanism underlying its toxic effect on the early development of vertebrates. BDE47-treated zebrafish larvae were found to present the light-related locomotion reduction in our previous study, therefore, we aimed to use high throughput sequencing to investigate the possible reasons from a transcriptomic perspective.</p></sec><sec><title>Results</title><p>By exposing zebrafish embryos/larvae to 5 μg/l and 500 μg/l BDE47, we measured the influence of BDE47 on the mRNA expression profiles of zebrafish larvae until 6 days post-fertilization, using Illumina HiSeq 2000 sequencing. Differential expression analysis and gene enrichment analysis respectively revealed that a great number of genes, and gene sets based on two popular terminologies, were affected by the treatment of 500 μg/l BDE47. Among them, BDE47 caused changes in the retinal metabolism and related biological processes involving eye morphogenesis and visual perception, as confirmed by disordered photoreceptor arrangement and thickened bipolar cell layer of larval retina from histological observations. Other altered genes such as <italic>pth1a</italic> and collaborative cathepsin family exhibited disrupted bone development, which was consistent with the body curvature phenotype. The transcriptome of larvae was not significantly affected by the treatment of 5 μg/l BDE47, as well as the treatment of DMSO vehicle.</p></sec><sec><title>Conclusions</title><p>Our results suggest that high BDE47 concentrations disrupt the eye and bone development of zebrafish larvae based on both transcriptomic and morphological evidences. The abnormal visual perception may result in the alteration of dark adaption, which was probably responsible for the abnormal larval locomotion. Body curvature arose from enhanced bone resorption because of the intensive up-regulation of related genes. We also proposed the larval retina as a novel potential target tissue for BDE47 exposure.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s12864-014-1194-5) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chernyak, Sm" uniqKey="Chernyak S">SM Chernyak</name></author><author><name sortKey="Rice, Cp" uniqKey="Rice C">CP Rice</name></author><author><name sortKey="Quintal, Rt" uniqKey="Quintal R">RT Quintal</name></author><author><name sortKey="Begnoche, Lj" uniqKey="Begnoche L">LJ Begnoche</name></author><author><name sortKey="Hickey, Jp" uniqKey="Hickey J">JP Hickey</name></author><author><name sortKey="Vinyard, Bt" uniqKey="Vinyard B">BT Vinyard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carlsson, P" uniqKey="Carlsson P">P 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Vaes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alt, B" uniqKey="Alt B">B Alt</name></author><author><name sortKey="Reibe, S" uniqKey="Reibe S">S Reibe</name></author><author><name sortKey="Feitosa, Nm" uniqKey="Feitosa N">NM Feitosa</name></author><author><name sortKey="Elsalini, Oa" uniqKey="Elsalini O">OA Elsalini</name></author><author><name sortKey="Wendl, T" uniqKey="Wendl T">T Wendl</name></author><author><name sortKey="Rohr, Kb" uniqKey="Rohr K">KB Rohr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Holtrop, Me" uniqKey="Holtrop M">ME Holtrop</name></author><author><name sortKey="Raisz, Lg" uniqKey="Raisz L">LG Raisz</name></author><author><name sortKey="Simmons, Ha" uniqKey="Simmons H">HA Simmons</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Lk" uniqKey="Wang L">LK Wang</name></author><author><name sortKey="Feng, Zx" uniqKey="Feng Z">ZX Feng</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author><author><name sortKey="Wang, Xw" uniqKey="Wang X">XW Wang</name></author><author><name sortKey="Zhang, Xg" uniqKey="Zhang X">XG Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, Xx" uniqKey="Zhou X">XX Zhou</name></author><author><name sortKey="Wu, Ww" uniqKey="Wu W">WW Wu</name></author><author><name sortKey="Li, H" uniqKey="Li H">H Li</name></author><author><name sortKey="Cheng, Ym" uniqKey="Cheng Y">YM Cheng</name></author><author><name sortKey="Wei, N" uniqKey="Wei N">N Wei</name></author><author><name sortKey="Zong, J" uniqKey="Zong J">J Zong</name></author><author><name sortKey="Feng, Xy" uniqKey="Feng X">XY Feng</name></author><author><name sortKey="Xie, Zq" uniqKey="Xie Z">ZQ Xie</name></author><author><name sortKey="Chen, D" uniqKey="Chen D">D Chen</name></author><author><name sortKey="Manley, Jl" uniqKey="Manley J">JL Manley</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H Wang</name></author><author><name sortKey="Feng, Y" uniqKey="Feng Y">Y Feng</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Xu, Ting" sort="Xu, Ting" uniqKey="Xu T" first="Ting" last="Xu">Ting Xu</name></noRegion><name sortKey="Dong, Bingzhi" sort="Dong, Bingzhi" uniqKey="Dong B" first="Bingzhi" last="Dong">Bingzhi Dong</name><name sortKey="Xu, Ting" sort="Xu, Ting" uniqKey="Xu T" first="Ting" last="Xu">Ting Xu</name><name sortKey="Yin, Daqiang" sort="Yin, Daqiang" uniqKey="Yin D" first="Daqiang" last="Yin">Daqiang Yin</name><name sortKey="Zhao, Jing" sort="Zhao, Jing" uniqKey="Zhao J" first="Jing" last="Zhao">Jing Zhao</name><name sortKey="Zhao, Qingshun" sort="Zhao, Qingshun" uniqKey="Zhao Q" first="Qingshun" last="Zhao">Qingshun 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